Season 16 of Supernatural is not confirmed, but it seems Ackles and Padalecki would be down for it. While speaking at the 2024 Creation Honolulu convention, both actors confirmed that there are talks of more Supernatural.

We all came to understand what the guys wanted to do,  Pedowitz said, explaining that Ackles and Padalecki wanted to go out while the show was still relevant.  Additionally, Pedowitz cited spending time with their families and seeing what else was out in the world  as the guys' reasons for ending the show.