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Darkness is open to interpretation, since it's defined as a lack of light. There's also a figurative darkness, which can represent wickedness, sadness, ignorance, or simply mystery: "The details of their adventures would remain in darkness, unless one of them wrote a book someday."
Darkness can encompass a primitive chaos, and the powers of that chaos; it is not essentially evil, yet it is largely associated with death and destruction, captivity and spiritual darkness. The gloom which preceded the Fiat Lux traditionally represents the states that give rise to chaos.